Journal article
Microfluidic paper-based analytical device for the determination of nitrite and nitrate
BM Jayawardane, S Wei, ID McKelvie, SD Kolev
Analytical Chemistry | Published : 2014
DOI: 10.1021/ac5013249
Abstract
A low-cost disposable colorimetric microfluidic paper-based analytical device (μPAD) was developed for the determination of nitrite and nitrate. Nitrite is determined directly by the Griess reaction while nitrate is first reduced to nitrite in a hydrophilic channel of the μPAD with immobilized zinc microparticles. This μPAD is fabricated by a simple and inexpensive inkjet printing method. Under optimal conditions, the limits of detection and quantification for nitrite are 1.0 and 7.8 μM, respectively, while the corresponding values for nitrate are 19 and 48 μM, respectively. The repeatability, expressed as relative standard deviation (RSD), is less than 2.9% and 5.6% (n ≥ 8) for the determin..
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Awarded by Australian Research Council
Funding Acknowledgements
The authors would like to thank the Australian Research Council for financial support of this research (Grant DP1094179), Hercules Australia for providing the paper sizing agent, and Ms. Xu Li and Mr. David Ballerini, both from Monash University, for valuable assistance with the printing of the mu PAD. B.M.J. is grateful for receiving a research scholarship from The University of Melbourne.